WBA, WBO, IBF, and IBO holder outclassed challenger, Kubrat Pulev Sport with a hard right hand in round nine.
The Unified world heavyweight champion with a mix of power and patience knocked out Pulev to bolster hopes that a historic fight against Tyson Fury could soon be a reality.
To knock out the Bulgarian, the British heavyweight smashed home a right hand, forcing his mandatory challenger to turn his back and face a count in round three, before drilling him to the canvas with an uppercut.
Perhaps fatigued by the chaos, Joshua stepped off the gas and allowed Pulev to at least offer some mild threat, but a barrage of uppercuts in the ninth dropped Pulev and a straight right hand wiped him out seconds later.
The fight was watched by 1,000 fans granted access to Wembley Arena. There was a blend of the boxing skills and crushing punches on display that have helped build Joshua into the attraction he is.
Anthony Joshua speaks on the prospective Tyson Fury clash
After the fans roared at the prospect of the IBF, WBA and WBO champion facing WBC champion Fury next, Joshua, said he is ready for any challenger.
“I started this game in 2013, gathered the belts.
“Whoever has the belts I want to compete with. If that is Tyson Fury, let it be Tyson Fury.”
Reacting to the comment via social media, Fury said: “There you go everyone. Anthony Joshua got asked if he wanted the fight and he went round the bushes.
“I want the fight. I want the fight next. I will knock him out inside three rounds. I can’t wait to knock him out.”